Section: 802, (Thursday) Group: 4 Names: John Gibson, Kyle LaPlant Instructor: S. Royan, PhD Date: Nov 14, 2024 University Of Massachusetts, Lowell, Fall 2024 Immunology Lab Report 5: Bio-Rad Microassay for Immunoglobulin G Protein Concentration Contributions: Objective Gibson Material/Method Gibson Data/Result a) c) LaPlant; b) d) e) Gibson Discussion a)-d) Gibson Reference LaPlant Objective This experiment aimed to use Bio-Rad Microassay Coomassie Brilliant Blue G250 dye to measure the concentration of isolated rabbit immunoglobulin G (IgG) from DEAE (diethylethanolamine) Affi-Gel® eluate fractions from a previous experiment. Bovine serum albumin (BSA) protein concentration slope equation was obtained with the same dye at the same time as the IgG reaction with Coomassie Brilliant Blue G250 dye. IgG quantities for all fractions were calculated based on the BSA protein quantity slope equation. Finally, IgG concentrations for all fractions were calculat...