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  Section: 802, (Thursday) Group: 4 Names: John Gibson Instructor: S. Royan, PhD Date: Dec 06, 2024 University Of Massachusetts, Lowell, Fall 2024 Immunology Lab Report 7:  Severe Acute Respiratory Syndrom Antibody Diagnosis And Blood Typing Objective The experiment aimed to diagnose two patients, Patients A and B, for SARS-Cov viral infection serum antibody presentation of the Severe Acute Respiratory Syndrome (SARS) using indirect enzyme-linked immunosorbent assay (indirect ELISA). Also, this experiment aimed to give blood ABO group typing and Rhesus (Rh) group typing of other separate four simulated patients, Patients 1 through 4, with synthetic blood samples.  Material And Methods (4) ELISA Antibody Tests A flat-bottom microtiter array strip with 12 wells was utilized for indirect ELISA assay. Each well was loaded with 50 μL of purified, non-infectious SARS-Cov antigen particles (produced from human cell culture infected with SARS-Cov) to coat the wells at room ...
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  Section: 802, (Thursday) Group: 4 Names: John Gibson, Kyle LaPlant Instructor: S. Royan, PhD Date: Nov 21, 2024 University Of Massachusetts, Lowell, Fall 2024 Immunology Lab Report 6: Cytotoxicity Assay on Lymphocytes Isolated from Mouse Contributions:  Objective LaPlant Material/Method LaPlant Data/Result Gibson; Revision LaPlant Discussion a) e) LaPlant; b) c) d) Gibson Reference LaPlant & Gibson Objective The experiment aimed to isolate lymphocytes from the thymus and spleen of a dissected mouse and perform a cytotoxicity assay using Cedarlane’s anti-mouse CD-90.2 (Thy 1.2) monoclonal antibodies alongside complement component proteins. Performing the assay and gathering cell viability via hemocytometer allowed for the evaluation of cytotoxicity of Thy 1.2 and quantification of the number of T-cells in the thymus and spleen. Material And Methods (6) Isolation of Lymphocytes from Mouse Spleen and Thymus A euthanized CD1 strain mouse was placed in...
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